This Gordon-style conference emphasizes how basis and applied efforts in stem cell biology and engineering can combine to aid in the development of stem cell therapeutics and bioprocesses. By bringing together the bioengineering and stem cell biology communities we will be able to accelerate progress towards innovative solutions to basic and translation problems in regenerative medicine. Topics emphasize how quantitative approaches can yield an increased understanding of the biological mechanisms that underlie these stem cell fate choices, cancer stem cells, iPS cells, technologies to study stem cell function, and the development of bioprocesses to culture stem cells for commercial applications.