Light Microscopy

Zeiss Axio Observer Z1 1

Description
Inverted Fluorescent (DIC, Phase, GFP, DsRed, DAPI, Cy5), definite focus, environmental chamber, Metamorph software, cameras: Evolve EMCCD, Cool Snap HQ2.

Location
Longwood CLS-517D

Zeiss Axio Observer Z1 2

Description
Inverted Fluorescent (DIC, 100xPhase, GFP, Cy3) with 5 ports, optovar (1.6x and 2.5x) Stage top chamber, definite focus, Metamorph software, cameras: Nuance, and Hamamatsu 9100-02 EMCCD.

Location
Longwood CLS-519B

Zeiss Imager Z2

Description
Upright Fluorescent (DIC, DAPI, GFP, Cy3.5, Cy5), Metamorph software, cameras: Nuance, Hamamatsu Orca-R2, and MicroPublisher 5.0 color. 10x dry, 20x dry, 20x dipping water, 40x dipping water, 40x oil, 63x oil, and 100x oil objectives.

Location
Longwood CLS-519A

Leica TIRF on DM16000B

Description
405nm, 488nm, 561nm, and 635nm lasers for TIRF; widefield: DIC, DAPI, GFP, TexasRed, Cy3/Cy5, DAPI/FITC/Cy3/Cy5.

Location
Longwood CLS-528E

Leica SP5 X MP Inverted Confocal Microscope

Description
Leica SP5 X MP Inverted Laser Scanning Confocal Microscope with AOBS, five internal detectors (two HyD detectors and three PMTs), one NDD TL detector and four external NDD detectors.

• Equipped with Ludin incubation system with temperature and active CO2 control
• Single photon lasers: 405nm laser, Argon laser (458,476,488, 496 and 514nm), white laser (470-670nm), tandem scanning system
• Multiphoton laser: IR laser Chameleon Vision 2, 680-1080nm with precompensation
• Objectives: 5x/0.15 dry, 10x/0.40 dry, 20x/0.7 dry, 20◊/0.7 Imm/Corr, 25x/0.95 water, 40x/1.1 water,  40x/1.25-0.75 oil, 63x/1.4-0.60 oil, 63x/1.2 W/Corr, and 63x/1.30 Gly/Corr

Location
Longwood CLS-525E

Upright Zeiss LSM 710 NLO ready

Description
Axio Imager Z2 with Colibri FL Illumination and CoolSNAP HQ2 camera, LSM 710 with 34 spectral ch, lasers – 405, 458, 488, 514, 561 & 633nm, NLO ready.

Location
Cambridge, 58 Oxford, Rm 107