Duties & Responsibilities
As part of the Bioinspired Therapeutics & Diagnostics platform, we are currently seeking a research assistant that will significantly contribute to a research program funded by the Bill and Melinda Gates Foundation involving the use of microfluidic human small intestine in vitro models to study environmental enteric dysfunction and the role the microbiome and immune component. The successful candidate will be expected to manage intestinal organoid cultures as a liaison with Harvard Digestive Diseases Core facility and have the ability to plan and execute scientific research dealing with microfluidic Organ on Chip primary cell culture, microbiome culture, and the development and validation of various in-vitro cell based assays, including, but not limited to, cell based qPCR/TaqMan, ELISA, westerns, enzymatic assays, imaging, and flow cytometry. Previous experience with primary mammalian cell culture is a must, and previous experience with intestinal organoid culture is a significant plus. The candidate will be expected to work independently with little guidance from academic staff to design experiments, learn new skills, troubleshoot, and to contribute intellectually to the project. In addition, the candidate is expected to order supplies, perform support activities and lab maintenance, and function in a team-oriented environment with other scientists and staff at the Institute. The candidate will participate in the preparation of manuscripts and patent applications. Under the supervision of staff, the candidate may present data and conclusions at meetings within the Wyss Institute.
Bachelor’s degree in cell biology, bioengineering or related field with at least three years of experience involving aseptic primary human cell culture and molecular biology techniques, such as ELISA, PCR, microscopy or related experience. Advanced degree may count toward experience.
Candidates with experience in multiple primary cell types, specifically in gut organoid culture, will be strongly considered. Experience with cell culture in microfluidic device is a plus. Ability to work well independently and to collaborate with a multidisciplinary group is essential. The candidate should be proficient with personal computers and applicable software packages (Microsoft Office). Good written and oral communication skills are essential. The candidate should be highly motivated, detail-oriented, organized, and able to take initiative.
This is a grant-funded position for one year with the possibility of renewal. [46075BR]