Molecular testing technologies continue to evolve at a rapid pace. The field of clinical microbiology is undergoing rapid change with novel molecular technologies cutting the time needed to diagnose infections, enabling more precise and appropriate individualized therapies, improving patient care and optimizing clinical outcome. This field is on the cusp of the largest change in its history. The 2015 Molecular Diagnostics for Infectious Disease conference will highlight several emerging and cutting-edge technologies and discuss some of the hurdles for their regulation and integration into clinical practice.