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Mapping Neuronal Identities and Connections in Neural Circuits by Light Microscope


Neural circuits, composed of intercellular connected neurons with distinct properties lay the physical foundation of any brain function. Identifying subtype and connections of individual neurons in a circuit is the key to understand how information is processed and propagated in the brain. In order to obtain a detailed wiring diagram between neurons, we developed a series of multiplex labeling and profiling, sample preparation, and image processing tools to allow neuronal identity and connectivity features being directly measured by super-resolution light microscopy in a densely labeled mouse brain. Designed to be carried out with standard surgical, imaging and computational instrumentation, our comprehensive circuit mapping tool set will allow high-resolution, high-throughput neural circuit mapping in a regular neuroscience laboratory.

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