The talk will present novel self-assembling and printing technologies enabling the fabrication of 2D and 3D bioactive and/or biomimetic materials for potential application in tissue engineering, regenerative medicine, and in vitro models. Examples of projects that will be presented include a) a bioactive membrane capable of growing hierarchically-ordered hydroxyapatite structures that resemble those found in human dental enamel; b) a dynamic self-assembling peptide-protein system capable of controllably accessing non-equilibrium to grow robust tubes and capillaries with potential application in tissue engineering; and c) a simple 3D molecular printing method to create distinct chemical environments ranging from tens of microns to centimeters in size and depth within different types of hydrogels.